24 Pages Fermo_6.cdr
نویسندگان
چکیده
A method for regenerating pigeonpea {Cajanus cajan (L.) Millsp.} plants has been developed using cotyledonary nods and embroyonic axes of mature seeds as explants. A large number of shoot buds were induced directly from explants of genotypes Pusa-9 and Bahar when cultured on different basal media fortified with a range of BAP concentrations (0–2 mg/l) and 0–0.5 mg/l gibberellic acid (GA ) either individually or in combination. The 3 shoot buds developed into shoots when they were sub cultured on the same medium but with different concentrations of cytokinins. The shoots were elongated by sub culturing in Murashige and Skoog (MS) basal medium supplemented than elongated shoots transmitted to half-strength MS medium with 0.3–0.5 mg/l indole-3-butyric acid (IBA) for root induction. Frequency of shoot induction in embryonic axes was better to cotyledonary node. The frequency of shoot bud regeneration was influenced by genotype, type of explant and concentrations of growth hormones. This process may be useful for improving the crop through genetic manipulations.
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